Multi-ethnicSULT1A1copy number profiling with multiplex ligation-dependent probe amplification
نویسندگان
چکیده
منابع مشابه
Ligation-independent mechanism of multiplex ligation-dependent probe amplification.
Multiplex ligation-dependent probe amplification (MLPA) is a widely used technique for detecting genomic structural variants. The technique is based on hybridization and ligation, followed by amplification of the ligation products. Therefore, ligation is considered a fundamental process that determines the feasibility and fidelity of MLPA. However, despite the widespread use of this technique, ...
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Introduction: Lots of human diseases and syndromes result from partial or complete gene deletions and duplications or changes of certain specific chromosomal sequences. Many various methods are used to study the chromosomal aberrations including Comparative Genomic Hybridization (CGH), Fluorescent in Situ Hybridization (FISH), Southern blots, Multiplex Amplifiable Probe Hybridisation (MAP...
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PURPOSE Metastasis from uveal melanoma occurs almost exclusively with tumors showing chromosome 3 loss. We used multiplex ligation-dependent probe amplification (MLPA) to detect chromosome 1p, 3, 6p, 6q, 8p, and 8q abnormalities in uveal melanomas. The purpose of this study was to correlate our MLPA results with other risk factors and metastatic death. EXPERIMENTAL DESIGN Patients were includ...
متن کاملImaging, Diagnosis, Prognosis Genotypic Profiling of 452 Choroidal Melanomas with Multiplex Ligation-Dependent Probe Amplification
Purpose:Metastasis from uvealmelanoma occurs almost exclusively with tumors showing chromosome 3 loss.We usedmultiplex ligation-dependent probe amplification (MLPA) to detect chromosome 1p, 3, 6p, 6q, 8p, and 8q abnormalities in uveal melanomas. The purpose of this study was to correlate our MLPA results with other risk factors and metastatic death. Experimental Design: Patients were included i...
متن کاملMethylation-Specific Multiplex Ligation-Dependent Probe Amplification (MS-MLPA).
This chapter describes a method for the rapid assessment of promoter hypermethylation levels or methylation of imprinted regions in human genomic DNA extracted from various sources using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA). Multiplex ligation-dependent probe amplification (MLPA) is a powerful and easy-to-perform PCR-based technique that can identify g...
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ژورنال
عنوان ژورنال: Pharmacogenomics
سال: 2018
ISSN: 1462-2416,1744-8042
DOI: 10.2217/pgs-2018-0047